Effect of peracetic acid–ultraviolet combination treatment on microbial and endotoxin levels in a pharmaceutical water system

Abstract In the pharmaceutical industry, microbiological quality of water is vital. This research investigated how combining peracetic acid (0.1% V/V) and UV light (>150 mJ/cm2) as wide-spectrum disinfectants affect microbial endotoxin levels in a system. Water samples were taken aseptically from 12 points across The pour plate technique membrane filtration used for counts. presence distilled was by Limulus amebocyte lysate (LAL) test gel-clot method. After acid–UV combination treatment, counts significantly decreased (P < 0.05) compared with treatment alone, they lower than action limits specified European Pharmacopeia (100 CFU/ml purified 10 CFU/100 ml injection). addition, mainly LAL-negative (10 negative weekly reports out total reports). It concluded that disinfection all stages system remarkably improved Therefore, rotation between more one disinfectant policy periodic recommended to minimize contamination products well bacterial infections product consumers.